B--DNA SEQUENCING
Overview
Buyer
Place of Performance
NAICS
PSC
Set Aside
Original Source
Timeline
Qualification Details
Fit reasons
- NAICS alignment with historical contract wins in similar service areas.
- Scope strongly matches core technical capabilities and delivery model.
Risks
- Past performance thresholds may require one additional teaming partner.
- Potential clarification needed on staffing minimums before bid/no-bid.
Next steps
Validate eligibility requirements, assign capture owner, and schedule partner outreach to confirm teaming strategy before submission planning.
Quick Summary
The US Geological Survey (USGS) Eastern Ecological Science Center is conducting a Sources Sought for DNA sequencing services. This is for market research purposes only to identify potential sources capable of providing DNA sequencing of PCR amplicons from environmental DNA (eDNA) extracted from filtered seawater samples. The goal is to identify taxonomic composition. This notice is not a request for proposal, and no contract will be awarded from it. Responses are due by May 25, 2026.
Scope of Work
The requirement involves DNA sequencing of PCR amplicons, ranging between 100-400 bp, derived from environmental DNA extracted from filtered seawater samples. The primary objective is to identify the taxonomic composition of these samples.
Key Requirements
- Technology: Sequencing must be conducted on an Illumina NovaSeq 6000 using a 2x250 bp configuration on SP flowcells to maintain analytical consistency.
- Quality: A minimum of 75% of bases must be ≥Q30.
- Experience: The provider must have 15 or more years of experience in genomic shotgun and amplicon sequencing services.
- Track Record: A demonstrated record of project completion through sequencing environmental DNA amplicons on Illumina NovaSeq 6000 SP flowcells, including for federal government research laboratories, is required.
- Operational: The provider must perform 100% of the requested sequencing service in-house. This includes performing quality control (QC) on provided sequencing libraries and notifying the principal investigator within 72 hours of receiving damaged libraries or if input material fails QC. Demultiplexed fastq files must be provided after each run with sufficient time for data download.
Submission Details
- Response Due: May 25, 2026, via email to Mindie Dixon (mindie_dixon@ios.doi.gov).
- Content: Responses shall include:
- The extent to which each of the specified requirements can be met.
- Business type (commercial, academia), size (large/small), and any small disadvantaged status (HUBZone, 8(a), SDVOSB, WOSB, EDWOSB).
- Description of completed projects demonstrating relevant experience.
- Eligibility: Respondents must have a UEI number and be registered in SAM.gov.
Set-Aside & NAICS
- Set-Aside: This Sources Sought highly encourages participation from Small Business, Section 8(a), HUBZone, Service-Disabled Veteran-Owned Small Business (SDVOSB), Women-Owned Small Business (WOSB), and Economically Disadvantaged Women-Owned Small Business (EDWOSB).
- NAICS: 541380 (Testing Laboratories and Services), Size Standard: $19.0 million.
Additional Notes
This is for market research only; respondents will not be notified of evaluation results. Any future solicitation will be posted separately on SAM.gov, and potential responders are responsible for monitoring the site.