DNA Extraction and Shotgun Metagenomics Sequencing of Fecal Samples
Overview
Buyer
Place of Performance
NAICS
PSC
Set Aside
Original Source
Timeline
Qualification Details
Fit reasons
- NAICS alignment with historical contract wins in similar service areas.
- Scope strongly matches core technical capabilities and delivery model.
Risks
- Past performance thresholds may require one additional teaming partner.
- Potential clarification needed on staffing minimums before bid/no-bid.
Next steps
Validate eligibility requirements, assign capture owner, and schedule partner outreach to confirm teaming strategy before submission planning.
Quick Summary
The National Institutes of Health (NIH), under the Department of Health and Human Services, has issued a Notice of Intent to award a sole-source contract to Rush University Medical Center for DNA Extraction and Shotgun Metagenomics Sequencing of Fecal Samples. This is not a Request for Proposal. The acquisition is for research purposes, specifically to understand cross-species validation of mechanisms related to bile acid- and FXR-induced increases in ketogenesis. Capability statements from other responsible sources are due by May 23, 2026.
Scope of Work
The required services include:
- DNA extraction and shotgun metagenomics sequencing for a total of 200 fecal samples. This comprises:
- Human samples (n=25 at baseline, n=100 after ketogenic diet with/without NR supplementation).
- Mouse samples (n=25 at baseline, n=100 after high-fat diet with/without NR supplementation).
- Library preparation and sequencing for all 200 samples.
- Providing raw data files to the Principal Investigator, Dr. Katherine Maki. The services are for a "Keto Diet" project (Project ID: GMCF-3719).
Contract & Timeline
- Type: Special Notice / Notice of Intent (Sole Source)
- Set-Aside: None (Sole source to Rush University Medical Center)
- Statutory Authority: 41 U.S.C. 253(c)(1) as implemented by FAR 6.302-1 (only one responsible source).
- Estimated Value: $24,242.00 (based on provided quote).
- Response Due: May 23, 2026, by 12:00 PM Eastern Standard Time.
- Published: May 13, 2026.
- Place of Performance: Bethesda, MD, United States.
Submission Details
Responsible sources capable of providing the required services may submit a capability statement via email to valerie.gregorio@nih.gov. The subject line must reference NOI-CC-OLAO-26-005551.
Additional Notes
This notice is for informational purposes only and does not constitute a solicitation. The government's determination not to compete this requirement, based on responses to this notice, is solely at its discretion. The provided quote details specific pricing for DNA isolation, shotgun library prep, and Illumina sequencing.